Diagnostic significance of malondialdehyde level and catalase activity evaluation in women with preterm labor
Objective. To determine diagnostic significance of combined evaluation of malondialdehyde level and catalase activity in the blood of pregnant women with threat and/or onset of preterm labor.Vysokikh M.Y., Tyutyunnik V.L., Kan N.E., Kurchakova T.A., Sukhanova I.A., Volodina M.A., Tarasova N.V., Tsvirkun D.V., Medzidova M.K., Arushanova A.G.
Subject and methods. The study involved 112 women 18 to 40 years old. Group I – 36 pregnant women with delivery between 22 to 36 weeks of gestation and within 7 days after the examination. Group II included 42 women with threatened preterm labor and subsequent delivery at term. Group III – 34 generally healthy pregnant women delivered at term. Spectrophotometric method was used to measure level of malondialdehyde (MDA) as a marker of lipid peroxidation and catalase enzymatic activity in maternal peripheral blood.
Results. It was shown that the enzymatic activity of catalase in plasma of women with realized preterm labor and women with threated preterm delivery with subsequent pregnancy retention was increased in comparison to control group. The same time at 28–32 weeks of gestation the quantity of women with catalase activity higher than 150 U/ml was significantly higher in group of women with threated preterm delivery with subsequent pregnancy retention then in early delivered women. The concentration of MDA in plasma of women with realized preterm labor at 22–27 weeks of gestation was significantly higher in comparison with control group. At term of 28–32 weeks the same tendency was observed but the difference was not significant. No significant difference in MDA level was observed between women with threated preterm delivery and subsequent delivery at term and control group.
Conclusion. The main finding of the study is that in pregnant women there are some systemic compensatory reactions intended to decrease the level of reactive oxygen species production by catalase in the situation of threated preliminary labor. Catalase activity reduction in the blood of pregnant women with realized preliminary labor can indicate the disturbances in reactive oxygen species production system as a result of mother and placenta adaptive resources attenuation. Thus, elevated level of MDA at 22–27 weeks of gestation and decreased activity of catalase at 28–32 weeks of gestation may be negative prognostic factors in the case of preterm labor threat.
Keywords
preterm labor
oxidative stress
malondialdehyde
catalase
References
1. Khodzhaeva Z.S., Dembovskaya S.V., Dobrokhotova Yu.E., Sichinava L.G., Yuzko A.M., Maltseva L.I., Serova O.F., Makarov I.O., Akhmadeeva E.N., Bashmakova N.V., Shmakov R.G., Klimenchenko N.I., Muminova K.T., Talibov O.B., Sukhikh G.T.. Drug therapy for preterm birth: Results of the international multicenter open-label Mystery study. Akusherstvo i ginekologiya/Obstetrics and Gynecology. 2016; (8): 37-43. (in Russian) http://dx.doi.org/10.18565/aig.2016.8.37-43
2. Savelyeva G.M., Shalina R.I., Kurtser M.A., Klimenko P.A., Sichinava L.G., Panina O.B., Plekhanova E.R., Vykhristyuk Yu.V., Lebedev E.V. Preterm birth as the most important problem of modern obstetrics. Akusherstvo i Ginekologiya/ Obstetrics and Gynecology. 2012; (8): 4-10. (in Russia)
3. Knuppel R.A., Hassan M.I., Mc Dermott J.J., Tucker M., Morrison J.C. Oxidative stress and antioxidants: preterm birtb and preterm infants. In: Morrison J. ed. Preterm birth – mother and child. In Tech; 2012: 125-50.
4. Romero R., Miranda J., Chaiworapongsa T., Korzeniewski S.J., Chaemsaithong P., Gotsch F. et al. Prevalence and clinical significance of sterile intra-amniotic inflammation in patients with preterm labor and intact membranes. J. Reprod. Immunol. 2014; 72(5): 458-74.
5. Menon R. Oxidative stress damage as a detrimental factor in preterm birth pathology. Front. Immunol. 2014; 5: 567. doi: 10.3389/fimmu.2014.00567.
6. Auten R.L., Davis J.M. Oxygen toxicity and reactive oxygen species: the devil is in the details. Pediatr. Res. 2009; 66(2): 121-7.
7. Siddiqui I.A., Jaleel A., Tamimi W., Al Kadri H.M. Role of oxidative stress in the pathogenesis of preeclampsia. Am. J. Obstet. Gynecol. 2010; 282(5): 469-74.
8. Ruder E.H., Hartman T.J., Goldman M.B. Impact of oxidative stress on female fertility. Curr. Opin. Obstet. Gynecol. 2009; 21(3): 219-22.
9. Mert I., Oruc A.S., Yuksel S., Cakar E.S., Buyukkagnici U., Karaer A., Danisman N. Role of oxidative stress in preeclampsia and intrauterine growth restriction. Obstet. Gynecol. Res. 2012; 38(4): 658-64.
10. Rorth M., Jensen .PK. Determination of catalase activity by means of the Clark oxygen electrode. Biochim. Biophys. Acta. 1967; 139(1): 171-3.
11. Jentzsch A.M., Bachmann H., Fürst P., Biesalski H.K. Improved analysis of malondialdehyde in human body fluids. Free Radic. Biol. Med. 1996; 20(2): 251-6.
12. Thompson L.P., Al-Hasan Y. Impact of oxidative stress in fetal programming. J. Pregnancy. 2012; 2012: 582748.
13. Berglund L. Transplacental amino acid transfer and its study using positron emission tomography. Ups. J. Med. Sci. 1990; 95(2): 95-115.
14. Griffiths S.K., Campbell J.P. Placental structure, function and drug transfer. Contin. Educ. Anaesth. Crit. Care Pain. 2015; 15(2): 84-9.
15. Madeleneau D., Buffat C., Mondon F., Grimault H., Rigourd V., Tsatsaris V. et al. Transcriptomic analysis of human placenta in intrauterine growth restriction. Pediatr. Res. 2015; 77(6):799-807.
Received 31.08.2016
Accepted 02.09.2016
About the Authors
Vysokikh Mikhail Y., PhD, the head of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology,Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: m_vysokikh@oparina4.ru
Tyutyunnik Victor L., PhD, MD, the head of the obstetric physiological department, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: tioutiounnik@mail.ru
Kan Natalia E., PhD, MD, the head of the obstetric department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79262208655. E-mail: kan-med@mail.ru
Kurchakova Tatiana A., the postgraduate student, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79035512626. E-mail: t_kurchakova@oparina4.ru
Sukhanova Julia A., scientific researcher of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: suhanova_julia@hotmail.com
Volodina Maria A., scientific researcher of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: m_volodina@oparina4.ru
Tarasova Nadezda V., scientific researcher of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: n_tarasova@oparina4.ru
Tsvirkun Dariya V., scientific researcher of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: darunyat@gmail.com
Medzidova Marzanat K., PhD candidate, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: marzana-m@yandex.ru
Arushanova Alena G., the postgraduate student, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79265517824. E-mail: a_aruschanova@oparina4.ru
For citations: Vysokikh M.Y., Tyutyunnik V.L., Kan N.E., Kurchakova T.A., Sukhanova I.A., Volodina M.A., Tarasova N.V., Tsvirkun D.V., Medzidova M.K., Arushanova A.G. Diagnostic significance of malondialdehyde level and catalase activity evaluation in women with preterm labor. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (4): 62-67.
http://dx.doi.org/10.18565/aig.2017.4.62-67
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