Prevalence of HPV types in patients with cervical intraepithelial neoplasia of different severity

Mzarelua G.M., Nazarova N.M., Nekrasova M.E., Sycheva E.G., Bourmenskaya O.V., Starodubtseva N.L., Asaturova A.V., Habas G.N., Pavlovich S.V.

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia
Objective. To study the prevalence of HPV (human papillomavirus) types in patients with squamous intraepithelial lesions (SIL) of different severity and cervical cancer in comparative perspective.
Subjects and methods. This prospective study included 145 women aged 18 to 49 years. The survey consisted of: collecting complaints, anamnesis, gynecological status, conducting extended colposcopy, molecular biological methods (multiplex PCR with detection results in real-time for quantification and typing of HPV 21 types), cytological examination, histological analysis of biopsy material. 5 groups were formed including 145 women: 31 patients (21,3%) – NILM (I group), 30 patients (20,6%) – chronic cervicitis (histologically confirmed) (group II), 37 patients (22,7%) – LSIL (III group), 31 patients (21,3%) – HSIL (IV group ) and 14 patients (9,6%) – cervical cancer (V group).
Results. The most significant risk factors for cervical intraepithelial lesions are: early sexual initiation, great number of sexual partners (>4), sexual transmitted infections (STI) history. The most frequent HPV types were 16, 31, 33 and 51 types. We demonstrated that HPV type 16 was significantly more prevalent in the group with HSIL and squamous cervical cancer compared to group II (chronic cervicitis) and group III (LSIL). HPV 31 and 33 types are more prevalent in the group with HSIL. According to our results, there is the prevalence of the group A9 HPV in women with LSIL, HSIL and cervical cancer compared to group II (chronic cervicitis).
Conclusion. Our results demonstrate the key role of A9 HPV group in the development of premalignant and malignant cervical disease. It should also be noted that in patients with LSIL in addition to A9 HPV group there is a trend to increase the incidence of HPV groups A7, A10, A5.

Keywords

human papillomavirus
chronic cervicitis
cervical intraepithelial neoplasia
cervical cancer
real time PCR

Supplementary Materials

  1. Table 1. Risk factors for development of SIL
  2. Table 2. Correlation analysis of the main risk factors for the development of SIL
  3. Table 3. Correlation analysis of the main risk factors and the presence of SIL of varying severity
  4. Table 4. Comparative data of SIL risk factor assessment by groups
  5. Table 5. Comparative assessment of the occurrence of groups and types of HPV
  6. Table 6. HPV groups at different degrees of epithelial involvement of the cervix
  7. Figure. The presence of HPV in different groups

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Received 21.12.2016

Accepted 17.02.2017

About the Authors

Mzarelua Guranda Merabovna, the postgraduate student of Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: mzareluag@mail.ru
Nazarova Nico Mirzoevna, MD, Ph.D., Senior Researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: grab2@yandex.ru
Nekrasova Maria Evgenyevna, the postgraduate student of Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: mashenka_90@mail.ru
Sycheva Elena Gennadyevna, physicial, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: el.bona@mail.ru
Burmenskaya Olga Vladimirovna, Senior Researcher, Researcher, Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health and of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Starodubtseva Natalia Leonidovna, Ph.D., Head of the Laboratory of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117497, Russia, Moscow, Ac. Oparina str.4. Tel.: +79164639867. E-mail: proteom.oparina4@gmail.com
Asaturova Alexandra Vjacheslavovna, Senior Researcher, 1st Pathology Department, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4
Khabas Gregory Nikolaevich, Ph.D., Head of the Department of combined and integrated methods of treatment of gynecological diseases, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: g_khabas@oparina4.ru
Pavlovich Stanislav Vladislavovich, Ph.D., docent, academic secretary of the Federal State Budget Institution, Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79161893718. E-mail: s_pavlovich@oparina4.ru

For citations: Mzarelua G.M., Nazarova N.M., Nekrasova M.E., Sycheva E.G., Bourmenskaya O.V., Starodubtseva N.L., Asaturova A.V., Habas G.N., Pavlovich S.V. Prevalence of HPV types in patients with cervical intraepithelial neoplasia of different severity. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (4): 94-100. (in Russian)
https://dx.doi.org/10.18565/aig.2018.4.94-100

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